Acts 4:36 identifies Barnabas as a Levite from Cyprus. His connection with the island later became important in the missionary activity of the early church.
Acts 11:19-20 records believers from Cyprus and Cyrene preaching in Antioch. During Paul's first missionary journey, Paul, Barnabas, and John Mark sailed to Cyprus and proclaimed the word in the synagogues at Salamis before crossing the island to Paphos.
At Paphos Paul confronted Elymas the magician and encountered the Roman proconsul Sergius Paulus. Acts 15:39 later records Barnabas and Mark returning to Cyprus after Barnabas and Paul separated.