William Brassey Hole was born in Salisbury, Wiltshire, in 1846. His father died during the cholera epidemic of 1849. The family subsequently moved to Edinburgh, where Hole spent most of his life and developed his artistic career.
Hole was educated at Edinburgh Academy. He initially trained as a civil engineer, but his principal interest was art. In 1869 he traveled to Italy and spent several months sketching. While in Rome, he met the Scottish artist Keeley Halswelle, whose encouragement helped strengthen his decision to pursue art professionally.
After returning to Edinburgh, Hole continued his artistic training and became closely associated with the Royal Scottish Academy. He won the Academy's Stuart Prize in 1870. He became an associate member in 1878 and was elected a full academician in 1889. He also developed a substantial reputation as an etcher.
History painting became an important part of Hole's career. In the 1890s he undertook major mural projects in Edinburgh. He decorated the chancel of St James' Church on Inverleith Row and was later commissioned to create the decorative scheme for the central hall of the Scottish National Portrait Gallery. His processional frieze there presents figures from Scotland's history.
Biblical subjects occupied Hole increasingly during the later part of his career. In 1901 he traveled to Palestine to study the setting of the life of Jesus. He used paints and a camera to record landscapes, buildings, people, and clothing that could inform his illustrations. The resulting watercolors were eventually published as The Life of Jesus of Nazareth.
Hole returned to Egypt and Palestine in 1912. This journey was connected with plans for a series of Old Testament watercolors. The larger project remained unfinished.
Hole died in Edinburgh in 1917. His career encompassed portraiture, history painting, murals, etching, book illustration, and biblical art. His extensive association with Edinburgh made him an important figure in Scottish artistic life despite his English birth.